Pure Cultures Lab Essay

Introduction Pure tillages ar made of only one case of organisms and can be used to sphere their properties. A method used to discriminate consummate(a) coatings is making a steak-plate, which is a dilution process in which culture is sprinkle over an agar-agar plate in a certain manner. Using a loop rod, culture was taken from the pipe and dragged across area 1 several(prenominal) time,of the agar. The agar was then turned 90, and the loop was flamed and cooled. Taking some culture from area 1, it was dragged over area two,and the like steps were done for areas 3 and 4.Another technique used was strewing-plate, where the same culture is diffuse over the agar plate victimisation a sterile L-shaped bent glass in rod.The rod was dipped in 95% ethyl alcohol and flamed to sterlize. The nutrient agar was then placed on the plate, and spread with rod. An environmental plate was used to shield the cultures of a random object, in our experiment, it was the visual lens of a microscope. A cotton wool swab was dipped into sterile water, and a random item of our choice was swabbed. afterwards miscellanying the swab back in the water, the contaminated water was applied to a spread plate.Results See attached banter All the plates were successful is isolating the pure cultures except the environmental. The reason for this may give birth been that there was no bacteria, receivable to the detail they had been recently cleaned. The slant agars were able to hoof it up on the bacteria to commemorate the growth. The vial that had bright yellow bacteria growing was M.leuteus, showing the successful isolation and identification. Other vials that had M.Letues and S.marcescenes had a very sparse shade of bacteria growth.Questions1. No because a when a broth culture is used, it has not been inoculated from a pure culture, the only look would be to use a streaking method or spread plate. A mix culture slant is hard to isolate, because bacteria is clumped toge ther, getting a single dependency is difficult. These may cause contamination to the bacteria during the inoculation period.2. If there was more culture in quadrant 4 than 3, it is due to the loop being dragged back into quadrant 1. The nutrient agar that was in 1 came back to 4, and showed more culture.